Publications
(*: corresponding author; +: equal contribution)
2021 - Present
53. Ng AHC, Hu H, Wang K, Scherler K, Warren SE, Zollinger DR, McKay-Fleisch J, Sorg K, Beechem JM, Ragaglia E, Lacy JM, Smith KD, Marshall DA, Bundesmann MM, López de Castilla D, Corwin D, Yarid N, Knudsen BS, Lu Y*^, Goldman JD*, Heath JR*. Organ-specific immunity: A tissue analysis framework for investigating local immune responses to SARS-CoV-2. Cell Rep. 2023;42(10):113212. doi: 10.1016/j.celrep.2023.113212. PubMed PMID: 37792533. ^Lead contact.
52. Foy SP+, Jacoby K+, Bota DA+, Hunter T, Pan Z, Stawiski E, Ma Y, Lu W, Peng S, Wang CL, Yuen B, Dalmas O, Heeringa K, Sennino B, Conroy A, Bethune MT, Mende I, White W, Kukreja M, Gunturu S, Humphrey E, Hussaini A, An D, Litterman AJ, Quach BB, Ng AHC, Lu Y, Smith C, Campbell KM, Anaya D, Skrdlant L, Huang EY, Mendoza V, Mathur J, Dengler L, Purandare B, Moot R, Yi MC, Funke R, Sibley A, Stallings-Schmitt T, Oh DY, Chmielowski B, Abedi M, Yuan Y, Sosman JA, Lee SM, Schoenfeld AJ, Baltimore D, Heath JR, Franzusoff A, Ribas A*, Rao AV*, Mandl SJ*. Non-viral precision T cell receptor replacement for personalized cell therapy. Nature. 2023;615(7953):687-96. doi: 10.1038/s41586-022-05531-1. PubMed PMID: 36356599.
51. Chour W+, Choi J+, Xie J, Chaffee ME, Schmitt TM, Finton K, DeLucia DC, Xu AM, Su Y, Chen DG, Zhang R, Yuan D, Hong S, Ng AHC, Butler JZ, Edmark RA, Jones LC, Murray KM, Peng S, Li G, Strong RK, Lee JK, Goldman JD, Greenberg PD, Heath JR*. Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis. Commun Biol. 2023;6(1):528. doi: 10.1038/s42003-023-04899-8. PubMed PMID: 37193826.
50. Lu Y+, Ng AHC+, Chow FE, Everson RG, Helmink BA, Tetzlaff MT, Thakur R, Wargo JA, Cloughesy TF, Prins RM, Heath JR*. Resolution of tissue signatures of therapy response in patients with recurrent GBM treated with neoadjuvant anti-PD1. Nat Commun. 2021;12(1):4031. doi: 10.1038/s41467-021-24293-4. PubMed PMID: 34188042.
2016 - 2020
49. Wells DK+*, van Buuren MM+, Dang KK+, Hubbard-Lucey VM, Sheehan KCF, Campbell KM, Lamb A, Ward JP, Sidney J, Blazquez AB, Rech AJ, Zaretsky JM, Comin-Anduix B, Ng AHC, Chour W, Yu TV, Rizvi H, Chen JM, Manning P, Steiner GM, Doan XC, Merghoub T, Guinney J, Kolom A, Selinsky C, Ribas A, Hellmann MD, Hacohen N, Sette A, Heath JR, Bhardwaj N, Ramsdell F, Schreiber RD, Schumacher TN, Kvistborg P, Defranoux NA*. Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction. Cell. 2020;183(3):818-34.e13. doi: 10.1016/j.cell.2020.09.015. PubMed PMID: 33038342.
48. Su Y+, Ko ME+, Cheng H, Zhu R, Xue M, Wang J, Lee JW, Frankiw L, Xu A, Wong S, Robert L, Takata K, Yuan D, Lu Y, Huang S, Ribas A, Levine R, Nolan GP, Wei W, Plevritis SK, Li G*, Baltimore D*, Heath JR*. Multi-omic single-cell snapshots reveal multiple independent trajectories to drug tolerance in a melanoma cell line. Nat Commun. 2020;11(1):2345. doi: 10.1038/s41467-020-15956-9. PubMed PMID: 32393797.
47. Du J+, Su Y+, Qian C, Yuan D, Miao K, Lee D, Ng AHC, Wijker RS, Ribas A, Levine RD, Heath JR*, Wei L*. Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells. Nat Commun. 2020;11(1):4830. doi: 10.1038/s41467-020-18376-x. PubMed PMID: 32973134.
46. Yang G*, Lu Y, Bomba HN, Gu Z*. Cysteine-rich Proteins for Drug Delivery and Diagnosis. Curr Med Chem. 2019;26(8):1377-88. doi: 10.2174/0929867324666170920163156. PubMed PMID: 28933301.
45. Su Y, Bintz M, Yang Y, Robert L, Ng AHC, Liu V, Ribas A, Heath JR*, Wei W*. Phenotypic heterogeneity and evolution of melanoma cells associated with targeted therapy resistance. PLoS Comput Biol. 2019;15(6):e1007034. doi: 10.1371/journal.pcbi.1007034. PubMed PMID: 31166947.
44. Peng S+, Zaretsky JM+, Ng AHC+, Chour W+, Bethune MT, Choi J, Hsu A, Holman E, Ding X, Guo K, Kim J, Xu AM, Heath JE, Noh WJ, Zhou J, Su Y, Lu Y, McLaughlin J, Cheng D, Witte ON, Baltimore D, Ribas A, Heath JR*. Sensitive Detection and Analysis of Neoantigen-Specific T Cell Populations from Tumors and Blood. Cell Rep. 2019;28(10):2728-38.e7. doi: 10.1016/j.celrep.2019.07.106. PubMed PMID: 31484081.
43. Ng AHC+, Peng S+, Xu AM+, Noh WJ, Guo K, Bethune MT, Chour W, Choi J, Yang S, Baltimore D, Heath JR*. MATE-Seq: microfluidic antigen-TCR engagement sequencing. Lab Chip. 2019;19(18):3011-21. doi: 10.1039/c9lc00538b. PubMed PMID: 31502632.
42. Lu Y+, Yoo BK+*, Ng AHC, Kim J, Yeom S, Tang J, Lin MM, Zewail AH, Heath JR*. 4D electron microscopy of T cell activation. Proc Natl Acad Sci U S A. 2019;116(44):22014-9. doi: 10.1073/pnas.1914078116. PubMed PMID: 31611413.
41. Zhang Y, Wang J*, Yu J, Wen D, Kahkoska AR, Lu Y, Zhang X, Buse JB, Gu Z*. Bioresponsive Microneedles with a Sheath Structure for H₂O₂ and pH Cascade-Triggered Insulin Delivery. Small. 2018;14(14):e1704181. doi: 10.1002/smll.201704181. PubMed PMID: 29479811.
40. Yu S+, Wang C+, Yu J, Wang J, Lu Y, Zhang Y, Zhang X, Hu Q, Sun W, He C*, Chen X*, Gu Z*. Injectable Bioresponsive Gel Depot for Enhanced Immune Checkpoint Blockade. Adv Mater. 2018;30(28):e1801527. doi: 10.1002/adma.201801527. PubMed PMID: 29786888.
39. Yan J, Lu Y, Chen G, Yang M*, Gu Z*. Advances in liquid metals for biomedical applications. Chem Soc Rev. 2018;47(8):2518-33. doi: 10.1039/c7cs00309a. PubMed PMID: 29557433.
38. Ng AHC+, Fobel R+, Fobel C+, Lamanna J+, Rackus DG+, Summers A, Dixon C, Dryden MDM, Lam C, Ho M, Mufti NS, Lee V, Asri MAM, Sykes EA, Chamberlain MD, Joseph R, Ope M, Scobie HM, Knipes A, Rota PA, Marano N, Chege PM, Njuguna M, Nzunza R, Kisangau N, Kiogora J, Karuingi M, Burton JW, Borus P, Lam E, Wheeler AR*. A digital microfluidic system for serological immunoassays in remote settings. Sci Transl Med. 2018;10(438). doi: 10.1126/scitranslmed.aar6076. PubMed PMID: 29695457.
37. Chen Z+, Wang J+, Sun W, Archibong E, Kahkoska AR, Zhang X, Lu Y, Ligler FS, Buse JB, Gu Z*. Synthetic beta cells for fusion-mediated dynamic insulin secretion. Nat Chem Biol. 2018;14(1):86-93. doi: 10.1038/nchembio.2511. PubMed PMID: 29083418.
36. Lu Y+, Lin Y, Chen Z, Hu Q, Liu Y, Yu S, Gao W, Dickey MD*, Gu Z*. Enhanced Endosomal Escape by Light-Fueled Liquid-Metal Transformer. Nano Lett. 2017;17(4):2138-45. doi: 10.1021/acs.nanolett.6b04346. PubMed PMID: 28325042.
35. Lu Y, Gu Z*. Kidney physiology: A size bandpass filter. Nat Nanotechnol. 2017;12(11):1023-5. doi: 10.1038/nnano.2017.200. PubMed PMID: 28892100.
34. Hu X, Yu J, Qian C, Lu Y, Kahkoska AR, Xie Z, Jing X, Buse JB, Gu Z*. H₂O₂-Responsive Vesicles Integrated with Transcutaneous Patches for Glucose-Mediated Insulin Delivery. ACS Nano. 2017;11(1):613-20. doi: 10.1021/acsnano.6b06892. PubMed PMID: 28051306.
33. Abdulwahab S, Ng AHC, Chamberlain MD, Ahmado H, Behan LA, Gomaa H, Casper RF, Wheeler AR*. Towards a personalized approach to aromatase inhibitor therapy: a digital microfluidic platform for rapid analysis of estradiol in core-needle-biopsies. Lab Chip. 2017;17(9):1594-602. doi: 10.1039/c7lc00170c. PubMed PMID: 28379279.
32. Lu Y, Aimetti AA, Langer R*, Gu Z*. Bioresponsive materials. Nat Rev Mater. 2016;2(1):16075. doi: 10.1038/natrevmats.2016.75.
31. Shamsi MH, Choi K, Ng AHC, Chamberlain MD, Wheeler AR*. Electrochemiluminescence on digital microfluidics for microRNA analysis. Biosens Bioelectron. 2016;77:845-52. doi: 10.1016/j.bios.2015.10.036. PubMed PMID: 26516684.
30. Qian C+, Chen Y+, Zhu S, Yu J, Zhang L, Feng P, Tang X, Hu Q, Sun W, Lu Y, Xiao X, Shen QD*, Gu Z*. ATP-Responsive and Near-Infrared-Emissive Nanocarriers for Anticancer Drug Delivery and Real-Time Imaging. Theranostics. 2016;6(7):1053-64. doi: 10.7150/thno.14843. PubMed PMID: 27217838.
29. Hu Q, Sun W, Lu Y, Bomba HN, Ye Y, Jiang T, Isaacson AJ, Gu Z*. Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellular Drug-Delivery Depots. Nano Lett. 2016;16(2):1118-26. doi: 10.1021/acs.nanolett.5b04343. PubMed PMID: 26785163.
28. Dixon C, Ng AHC, Fobel R, Miltenburg MB, Wheeler AR*. An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays. Lab Chip. 2016;16(23):4560-8. doi: 10.1039/c6lc01064d. PubMed PMID: 27801455.
Before 2015
27. Sun W, Lu Y, Gu Z*. Rolling circle replication for engineering drug delivery carriers. Ther Deliv. 2015;6(7):765-8. doi: 10.4155/tde.15.27. PubMed PMID: 26228768.
26. Pacardo DB, Neupane B, Rikard SM, Lu Y, Mo R, Mishra SR, Tracy JB, Wang G, Ligler FS*, Gu Z*. A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment. Nanoscale. 2015;7(28):12096-103. doi: 10.1039/c5nr01568e. PubMed PMID: 26122945.
25. Ng AHC+, Chamberlain MD+, Situ H, Lee V, Wheeler AR*. Digital microfluidic immunocytochemistry in single cells. Nat Commun. 2015;6:7513. doi: 10.1038/ncomms8513. PubMed PMID: 26104298.
24. Ng AHC*, Wheeler AR*. Next-generation microfluidic point-of-care diagnostics. Clin Chem. 2015;61(10):1233-4. doi: 10.1373/clinchem.2015.240226. PubMed PMID: 25943113.
23. Ng AHC, Li BB, Chamberlain MD, Wheeler AR*. Digital Microfluidic Cell Culture. Annu Rev Biomed Eng. 2015;17:91-112. doi: 10.1146/annurev-bioeng-071114-040808. PubMed PMID: 26643019.
22. Ng AHC, Lee M, Choi K, Fischer AT, Robinson JM, Wheeler AR*. Digital microfluidic platform for the detection of rubella infection and immunity: a proof of concept. Clin Chem. 2015;61(2):420-9. doi: 10.1373/clinchem.2014.232181. PubMed PMID: 25512641.
21. Lu Y, Hu Q, Lin Y, Pacardo DB, Wang C, Sun W, Ligler FS, Dickey MD, Gu Z*. Transformable liquid-metal nanomedicine. Nat Commun. 2015;6:10066. doi: 10.1038/ncomms10066. PubMed PMID: 26625944.
20. Lafrenière NM, Mudrik JM, Ng AHC, Seale B, Spooner N, Wheeler AR*. Attractive design: an elution solvent optimization platform for magnetic-bead-based fractionation using digital microfluidics and design of experiments. Anal Chem. 2015;87(7):3902-10. doi: 10.1021/ac504697r. PubMed PMID: 25759941.
19. Weng Y, Lu Y, Gu Z*. Hydrogels for Drug Delivery. Perspectives in Micro- and Nanotechnology for Biomedical Applications. 2015;191-224. doi: 10.1142/9781783269617_0007
18. Tai W+, Mo R+, Lu Y, Jiang T, Gu Z*. Folding graft copolymer with pendant drug segments for co-delivery of anticancer drugs. Biomaterials. 2014;35(25):7194-203. doi: 10.1016/j.biomaterials.2014.05.004. PubMed PMID: 24875756.
17. Sun W, Lu Y, Gu Z*. Advances in Anticancer Protein Delivery Using Micro-/ Nanoparticles. Part Part Syst Charact. 2014;31(12):1204-22. doi: 10.1002/ppsc.201400140. PubMed PMID: 27642232.
16. Sun W, Jiang T, Lu Y, Reiff M, Mo R*, Gu Z*. Cocoon-like self-degradable DNA nanoclew for anticancer drug delivery. J Am Chem Soc. 2014;136(42):14722-5. doi: 10.1021/ja5088024. PubMed PMID: 25336272.
15. Shamsi MH+, Choi K+, Ng AHC, Wheeler AR*. A digital microfluidic electrochemical immunoassay. Lab Chip. 2014;14(3):547-54. doi: 10.1039/c3lc51063h. PubMed PMID: 24292705.
14. Lu Y, Sun W, Gu Z*. Stimuli-responsive nanomaterials for therapeutic protein delivery. J Control Release. 2014;194:1-19. doi: 10.1016/j.jconrel.2014.08.015. PubMed PMID: 25151983.
13. Lu Y, Mo R, Tai W, Sun W, Pacardo DB, Qian C, Shen Q, Ligler FS, Gu Z*. Self-folded redox/acid dual-responsive nanocarriers for anticancer drug delivery. Chem Commun. 2014;50(95):15105-8. doi: 10.1039/c4cc07004f. PubMed PMID: 25331017.
12. Fobel R, Kirby AE, Ng AHC, Farnood RR, Wheeler AR*. Paper microfluidics goes digital. Adv Mater. 2014;26(18):2838-43. doi: 10.1002/adma.201305168. PubMed PMID: 24458780.
11. Jin H, Lu Y, Ma P-P, Wu S-S*, Shen J. Structure and properties of irradiated HDPE high-density polyethylene/calcium carbonate composites. Journal of Thermoplastic Composite Materials. 2014;29(7):893--903. doi: 10.1177/0892705714551237.
10. Choi K+, Ng AHC+, Fobel R, Chang-Yen DA, Yarnell LE, Pearson EL, Oleksak CM, Fischer AT, Luoma RP, Robinson JM, Audet J, Wheeler AR*. Automated digital microfluidic platform for magnetic-particle-based immunoassays with optimization by design of experiments. Anal Chem. 2013;85(20):9638-46. doi: 10.1021/ac401847x. PubMed PMID: 23978190.
9. Lu Y, Ma P-P, Wu S-S, Chen Q, Shen J*. 微甲醛含量脲醛树脂的制备. 高分子材料科学与工程. 2013;29(2):128--30.
8. Ng AHC, Choi K, Luoma RP, Robinson JM, Wheeler AR*. Digital microfluidic magnetic separation for particle-based immunoassays. Anal Chem. 2012;84(20):8805-12. doi: 10.1021/ac3020627. PubMed PMID: 23013543.
7. Choi K, Ng AHC, Fobel R, Wheeler AR*. Digital microfluidics. Annu Rev Anal Chem. 2012;5:413-40. doi: 10.1146/annurev-anchem-062011-143028. PubMed PMID: 22524226.
6. Lu Y, Ma P-P, Wu S-S*, Shen J. Studies on fast functionalization of HDPE by ultraviolet irradiation and functionalized HDPE/CaCO3 composites. Polymer Bulletin. 2012;68(8):2089--96. doi: 10.1007/s00289-011-0666-x.
5. Miller EM, Ng AHC, Uddayasankar U, Wheeler AR*. A digital microfluidic approach to heterogeneous immunoassays. Anal Bioanal Chem. 2011;399(1):337-45. doi: 10.1007/s00216-010-4368-2. PubMed PMID: 21057776.
4. Lu Y, Zhou J, Shi H-P, Chen Q, Wu S-S*. 甲氧基聚乙二醇单醚丙烯酸酯的合成及共聚物的热稳定性. 高分子材料科学与工程. 2011;27(12):1--3.
3. Ng AHC+, Uddayasankar U+, Wheeler AR*. Immunoassays in microfluidic systems. Anal Bioanal Chem. 2010;397(3):991-1007. doi: 10.1007/s00216-010-3678-8. PubMed PMID: 20422163.
2. Jebrail MJ, Ng AHC, Rai V, Hili R, Yudin AK*, Wheeler AR*. Synchronized synthesis of peptide-based macrocycles by digital microfluidics. Angew Chem Int Ed Engl. 2010;49(46):8625-9. doi: 10.1002/anie.201001604. PubMed PMID: 20715231.
1. Jebrail MJ+, Luk VN+, Shih SC+, Fobel R, Ng AHC, Yang H, Freire SL, Wheeler AR*. Digital microfluidics for automated proteomic processing. J Vis Exp. 2009(33). doi: 10.3791/1603. PubMed PMID: 19898419.